Protocol to perform ex vivo electroretinography on adult zebrafish. STAR Protoc 2025 Mar 21;6(1):103565
Date
01/17/2025Pubmed ID
39823232Pubmed Central ID
PMC11786734DOI
10.1016/j.xpro.2024.103565Scopus ID
2-s2.0-85214862331 (requires institutional sign-in at Scopus site) 1 CitationAbstract
Ex vivo electroretinography (ERG) provides insight into the health and functionality of retinal cells, the integrity of phototransduction, and the visual cycle and allows for the direct application of pharmaceuticals to the retinal tissues. Here, we present a protocol for performing ex vivo ERG on adult zebrafish. We describe steps for zebrafish tissue dissection, mounting tissues, and assembling and connecting cassettes. We then detail procedures for running the Diagnosys software and processing and analyzing the resulting data.
Author List
Simpson SL, Collery RFAuthor
Ross F. Collery PhD Associate Professor in the Ophthalmology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsElectroretinography
Retina
Zebrafish
