Faculty Collaboration Database

Symplocos cochinchinensis enhances insulin sensitivity via the down regulation of lipogenesis and insulin resistance in high energy diet rat model. J Ethnopharmacol 2016 Dec 04;193:500-509

Date

10/01/2016

Pubmed ID

27686268

DOI

10.1016/j.jep.2016.09.050

Scopus ID

2-s2.0-84991736346 (requires institutional sign-in at Scopus site)   20 Citations

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE: This plant has been utilized in Indian system of medicine for treatment of diabetes. This is clearly evident from the composition of Ayurvedic preparation for diabetes 'Nisakathakadi Kashayam' where this is one of the main ingredients of this preparation AIM OF THE STUDY: The study aims in elucidating the molecular mechanisms underlying the insulin sensitizing effects of Symplocos cochinchinensis ethanol extract (SCE) using a high fructose and saturated fat (HFS) fed insulin resistant rat model.

MATERIALS AND METHODS: Experimental groups consisted of normal diet (ND), ND+SCE 500mg/kg bwd, HFS+vehicle, HFS+metformin 100mg/kg bwd, HFS+SCE 250/500mg/kg bwd. Initially the animals were kept under HFS diet for 8 weeks, and at the end of 8 week period, animals were found to develop insulin resistance and dyslipidemia. Post-administration of SCE, metformin or vehicle were carried out for 3 weeks. Gene and protein expressions relevant to insulin signalling pathway were analysed.

RESULTS: HFS significantly altered the normal physiology of animals via proteins and genes relevant to metabolism like stearoyl-CoA desaturase (SCD1), sterol regulatory element binding protein 1 (SREBP-1c), fatty acid synthase (FAS), glucose 6 phosphatase (G6Pase), phosphoenol pyruvate carboxykinase (PEPCK), glucose transporter 2 (GLUT2), protein tyrosine phosphatse 1B (PTP1B), peroxisome proliferator activated receptor alpha (PPAR alpha), sirtuin 1 (SIRT1) and glucokinase. SCE administration attenuates the insulin resistance in HFS rat by the down regulation of SCD1 gene expression that modulates SREBP-1c dependent and independent hepatic lipid accumulation.

CONCLUSION: SCE enhances insulin sensitivity via the down regulation of lipogenesis and insulin resistance in HFS rat model.

Author List

Antu KA, Riya MP, Nair A, Mishra A, Srivastava AK, Raghu KG

Author

Anupama Nair Postdoctoral Researcher 3 in the Obstetrics and Gynecology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Biomarkers
Blood Glucose
Diabetes Mellitus, Experimental
Diet, High-Fat
Dose-Response Relationship, Drug
Down-Regulation
Dyslipidemias
Ethanol
Fructose
Gene Expression Regulation, Enzymologic
Hypoglycemic Agents
Insulin
Insulin Resistance
Lipogenesis
Liver
Magnoliopsida
Male
Metformin
Phytotherapy
Plant Extracts
Plants, Medicinal
Rats, Sprague-Dawley
Solvents
Time Factors