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Membrane fluidity profiles as deduced by saturation-recovery EPR measurements of spin-lattice relaxation times of spin labels. J Magn Reson 2011 Oct;212(2):418-25

Date

08/27/2011

Pubmed ID

21868272

Pubmed Central ID

PMC3214655

DOI

10.1016/j.jmr.2011.07.022

Scopus ID

2-s2.0-84860390260   31 Citations

Abstract

There are no easily obtainable EPR spectral parameters for lipid spin labels that describe profiles of membrane fluidity. The order parameter, which is most often used as a measure of membrane fluidity, describes the amplitude of wobbling motion of alkyl chains relative to the membrane normal and does not contain explicitly time or velocity. Thus, this parameter can be considered as nondynamic. The spin-lattice relaxation rate (T(1)(-1)) obtained from saturation-recovery EPR measurements of lipid spin labels in deoxygenated samples depends primarily on the rotational correlation time of the nitroxide moiety within the lipid bilayer. Thus, T(1)(-1) can be used as a convenient quantitative measure of membrane fluidity that reflects local membrane dynamics. T(1)(-1) profiles obtained for 1-palmitoyl-2-(n-doxylstearoyl)phosphatidylcholine (n-PC) spin labels in dimyristoylphosphatidylcholine (DMPC) membranes with and without 50 mol% cholesterol are presented in parallel with profiles of the rotational diffusion coefficient, R(⊥), obtained from simulation of EPR spectra using Freed's model. These profiles are compared with profiles of the order parameter obtained directly from EPR spectra and with profiles of the order parameter obtained from simulation of EPR spectra. It is shown that T(1)(-1) and R(⊥) profiles reveal changes in membrane fluidity that depend on the motional properties of the lipid alkyl chain. We find that cholesterol has a rigidifying effect only to the depth occupied by the rigid steroid ring structure and a fluidizing effect at deeper locations. These effects cannot be differentiated by profiles of the order parameter. All profiles in this study were obtained at X-band (9.5 GHz).

Author List

Mainali L, Feix JB, Hyde JS, Subczynski WK

Authors

Jimmy B. Feix PhD Professor in the Biophysics department at Medical College of Wisconsin
Witold K. Subczynski PhD Professor in the Biophysics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Cholesterol
Data Interpretation, Statistical
Diffusion
Dimyristoylphosphatidylcholine
Electron Spin Resonance Spectroscopy
Lipid Bilayers
Membrane Fluidity
Membranes, Artificial
Nonlinear Dynamics
Phospholipids
Spin Labels