Evaluation of four methods for platelet compatibility testing. Blood 1987 May;69(5):1425-30
Date
05/01/1987Pubmed ID
3552075Scopus ID
2-s2.0-0023229411 (requires institutional sign-in at Scopus site) 31 CitationsAbstract
Four platelet compatibility assays were performed on serum and platelet or lymphocyte samples from 38 closely HLA-matched donor/recipient pairs involved in 55 single-donor platelet transfusions. The 22 patients studied were refractory to transfusions of pooled random-donor platelets. Of the four assays (platelet suspension immunofluorescence, PSIFT; 51Cr release; microlymphocytotoxicity; and a monoclonal anti-IgG assay, MAIA), the MAIA was most predictive of platelet transfusion outcome (predictability, 74% for one-hour posttransfusion platelet recovery and 76% for 24-hour recovery). The only other assay to reach statistical significance was the PSIFT (63% predictability for one-hour posttransfusion recovery). The degree of HLA compatibility between donor and recipient (exact matches v those utilizing cross-reactive associations) was unrelated to the ability of the MAIA to predict transfusion results. The MAIA may be capable of differentiating HLA antibodies, ABO antibodies, and platelet-specific antibodies responsible for failure of HLA-matched and selectively mismatched single-donor platelet transfusions.
Author List
McFarland JG, Aster RHMESH terms used to index this publication - Major topics in bold
Antibodies, MonoclonalBlood Donors
Blood Platelets
Cell Survival
Chromium Radioisotopes
Cross Reactions
Cytotoxicity Tests, Immunologic
Evaluation Studies as Topic
Fluorescent Antibody Technique
HLA Antigens
Histocompatibility Testing
Humans
Platelet Transfusion
