Biomimetic Composite Scaffold Containing Small Intestinal Submucosa and Mesoporous Bioactive Glass Exhibits High Osteogenic and Angiogenic Capacity. Tissue Eng Part A 2018 Jul;24(13-14):1044-1056
Date
01/20/2018Pubmed ID
29350101DOI
10.1089/ten.TEA.2017.0398Scopus ID
2-s2.0-85049621857 (requires institutional sign-in at Scopus site) 28 CitationsAbstract
Biomaterials with excellent osteogenic and angiogenic activities are desirable to repair massive bone defects. Decellularized matrix from porcine small intestinal submucosa (SIS) has attracted particular attention for tissue regeneration because it has strong angiogenic effects and retains plentiful bioactive components. However, it has inferior osteoinductivity and osteoconductivity. In this study, we developed porous composite of SIS combined with mesoporous bioactive glass (SIS/MBG) with the goal of improving the mechanical and biological properties. SIS/MBG scaffolds showed uniform interconnected macropores (∼150 μm), high porosity (∼76%), and enhanced compressive strength (∼0.87 MPa). The proliferation and osteogenic gene expression (Runx2, ALP, Ocn, and Col-Iα) of rat bone marrow stromal cells (rBMSCs) as well as the proliferation, angiogenic gene expression (VEGF, bFGF, and KDR), and tube formation capacity of human umbilical vein endothelial cells (HUVECs) in SIS/MBG scaffolds were significantly upregulated compared with nonmesoporous bioactive glass (BG)-modified SIS (SIS/BG) and SIS-only scaffolds. Western blot analysis revealed that SIS/MBG induced rBMSCs to osteogenic differentiation through the activation of Wnt/β-Catenin signaling pathway, and SIS/MBG enhanced angiogenic activity of HUVEC through the activation of PI3k/Akt pathways. The in vivo results demonstrated that SIS/MBG scaffolds significantly enhanced new bone formation and neovascularization simultaneously in critical-sized rat calvarial defects as compared with SIS/BG and SIS. Collectively, the osteostimulative and angiostimulative biomimetic composite scaffold SIS/MBG represents an exciting biomaterial option for bone regeneration.
Author List
Sun T, Liu M, Yao S, Ji Y, Xiong Z, Tang K, Chen K, Yang H, Guo XAuthor
Hu Yang PhD Chair, Professor in the Biomedical Engineering department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBiomimetic Materials
Bone Matrix
Calcification, Physiologic
Cell Differentiation
Cell Proliferation
Compressive Strength
Glass
Human Umbilical Vein Endothelial Cells
Humans
Intestinal Mucosa
Intestine, Small
Male
Neovascularization, Physiologic
Osteocalcin
Osteogenesis
Phosphatidylinositol 3-Kinases
Platelet Endothelial Cell Adhesion Molecule-1
Porosity
Proto-Oncogene Proteins c-akt
Rats, Sprague-Dawley
Skull
Swine
Tissue Scaffolds
Wnt Signaling Pathway
X-Ray Microtomography
