Evaluation of osteogenic inductivity of a novel BMP2-mimicking peptide P28 and P28-containing bone composite. J Biomed Mater Res A 2018 Jan;106(1):210-220
Date
09/09/2017Pubmed ID
28884509DOI
10.1002/jbm.a.36228Scopus ID
2-s2.0-85035083091 (requires institutional sign-in at Scopus site) 20 CitationsAbstract
We developed a novel BMP2-mimicking peptide P28, which possesses two distinct special structural elements-seven repetitive aspartic acids and phosphorylated serine designed to promote affinity to hydroxyapatite as well as nucleation of apatite crystallization and mineralization. In this work, we systematically studied the osteogenic inductivity of P28 in vitro and in vivo in comparison with BMP2. We confirmed its effects on proliferation, recruitment, and osteogenic differentiation of MC3T3-E1 cells in vitro. Furthermore, we loaded P28 into a biomimetic bone composite nanohydroxyapatite/collagen/poly(l-lactide) (nHAC/PLA) and evaluated its ability to promote bone regeneration in vivo using a rabbit femoral condyle defect model. The micro-CT reconstruction and quantification analysis and the histological analysis indicated that P28/nHAC/PLA bone composite was capable of repairing bone defects. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 210-220, 2018.
Author List
Sun T, Qu Y, Cui W, Yang L, Ji Y, Yu W, Navinduth R, Shao Z, Yang H, Guo XAuthor
Hu Yang PhD Chair, Professor in the Biomedical Engineering department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAspartic Acid
Biomimetic Materials
Bone Morphogenetic Protein 2
Bone Regeneration
Bone and Bones
Cell Differentiation
Cell Line
Collagen
Disease Models, Animal
Durapatite
Male
Mice
Osteoblasts
Osteogenesis
Peptides
Polyesters
Rabbits
Serine
Tissue Scaffolds
