Faculty Collaboration Database

Mildly Cross-Linked Dendrimer Hydrogel Prepared via Aza-Michael Addition Reaction for Topical Brimonidine Delivery. J Biomed Nanotechnol 2017 Dec;13(9):1089-1096

Date

02/27/2018

Pubmed ID

29479294

Pubmed Central ID

PMC5819351

DOI

10.1166/jbn.2017.2436

Scopus ID

2-s2.0-85038826979 (requires institutional sign-in at Scopus site)   31 Citations

Abstract

In this work, we developed a mildly cross-linked dendrimer hydrogel (mcDH) via aza-Michael addition of polyamidoamine (PAMAM) dendrimer G5 and polyethylene glycol diacrylate (PEG-DA, M_n=575 g/mol). We chose the antiglaucoma drug brimonidine tartrate as a model drug and developed a new antiglaucoma drug formulation on the basis of mcDH. Cytotoxicity of the mcDH formulation to NIH3T3 fibroblasts, in vitro drug release kinetics and ex vivo drug permeability across the rabbit cornea were examined. We also studied interactions between PAMAM dendrimer and the drug using ¹H NMR spectroscopy for a mechanistic understanding of brimonidine release from the mcDH. mcDH was found to be efficient unionizing brimonidine tartrate to form and encapsulate brimonidine free base for sustained release and enhanced corneal permeation.

Author List

Wang J, Williamson GS, Lancina Iii MG, Yang H

Author

Hu Yang PhD Chair, Professor in the Biomedical Engineering department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Brimonidine Tartrate
Dendrimers
Drug Liberation
Fibroblasts
Hydrogels
Mice
NIH 3T3 Cells
Rabbits