Click hybridization of immune cells and polyamidoamine dendrimers. Adv Healthc Mater 2014 Sep;3(9):1430-8
Date
02/28/2014Pubmed ID
24574321Pubmed Central ID
PMC4133313DOI
10.1002/adhm.201300515Scopus ID
2-s2.0-84908018516 (requires institutional sign-in at Scopus site) 26 CitationsAbstract
Immobilizing highly branched polyamidoamine (PAMAM) dendrimers to the cell surface represents an innovative method of enhancing cell surface loading capacity to deliver therapeutic and imaging agents. In this work, hybridized immune cells, that is, macrophage RAW264.7 (RAW), with PAMAM dendrimer G4.0 (DEN) on the basis of bioorthogonal chemistry are clicked. Efficient and selective cell surface immobilization of dendrimers is confirmed by confocal microscopy. Viability and motility of RAW-DEN hybrids remain the same as untreated RAW cells according to WST-1 assay and wound closure assay. Furthermore, Western blot analysis reveals that there are no significant alterations in the expression levels of signaling molecules AKT, p38, and NFκB (p65) and their corresponding activated (phosphorylated) forms in RAW cells treated with azido sugar and dendrimer, indicating that the hybridization process neither induced cell stress response nor altered normal signaling pathways. Taken together, this work shows the feasibility of applying bioorthogonal chemistry to create cell-nanoparticle hybrids and demonstrates the noninvasiveness of this cell surface engineering approach.
Author List
Xu L, Zolotarskaya OY, Yeudall WA, Yang HAuthor
Hu Yang PhD Chair, Professor in the Biomedical Engineering department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCell Movement
Cell Survival
Click Chemistry
Dendrimers
Macrophages
Materials Testing
Mice
Nanoparticles
Polyamines
Signal Transduction
