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Fli+ etsrp+ hemato-vascular progenitor cells proliferate at the lateral plate mesoderm during vasculogenesis in zebrafish. PLoS One 2011 Feb 25;6(2):e14732

Date

03/03/2011

Pubmed ID

21364913

Pubmed Central ID

PMC3045372

DOI

10.1371/journal.pone.0014732

Abstract

BACKGROUND: Vasculogenesis, the de novo formation of blood vessels from precursor cells is critical for a developing embryo. However, the signals and events that dictate the formation of primary axial vessels remain poorly understood.

METHODOLOGY/PRINCIPAL FINDINGS: In this study, we use ets-related protein-1 (etsrp), which is essential for vascular development, to analyze the early stages of vasculogenesis in zebrafish. We found etsrp(+) cells of the head, trunk and tail follow distinct developmental sequences. Using a combination of genetic, molecular and chemical approaches, we demonstrate that fli(+)etsrp(+) hemato-vascular progenitors (FEVPs) are proliferating at the lateral plate mesoderm (LPM). The Shh-VEGF-Notch-Hey2 signaling pathway controls the proliferation process, and experimental modulation of single components of this pathway alters etsrp(+) cell numbers at the LPM.

CONCLUSIONS/SIGNIFICANCE: This study for the first time defines factors controlling proliferation, and cell numbers of pre-migratory FEVPs in zebrafish.

Author List

Chun CZ, Remadevi I, Schupp MO, Samant GV, Pramanik K, Wilkinson GA, Ramchandran R

Author

Ramani Ramchandran PhD Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Blood Vessels
Cell Movement
Cell Proliferation
Embryo, Nonmammalian
Endothelium, Vascular
Gene Expression Regulation, Developmental
Head
Hematopoietic Stem Cells
Mesoderm
Models, Biological
Neovascularization, Physiologic
Proto-Oncogene Protein c-fli-1
Tail
Zebrafish
Zebrafish Proteins
jenkins-FCD Prod-484 8aa07fc50b7f6d102f3dda2f4c7056ff84294d1d