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Antiangiogenic activity of restin, NC10 domain of human collagen XV: comparison to endostatin. Biochem Biophys Res Commun 1999 Feb 24;255(3):735-9

Date

03/02/1999

Pubmed ID

10049780

DOI

10.1006/bbrc.1999.0248

Abstract

Based on a homology search with endostatin, the C-terminus 185 aa of collagen XVIII, we report the cloning, expression, and antiangiogenic activity of a 22 kDa human collagen XV fragment, that we have named restin. Restin was expressed in the prokaryotic pET expression system. We have shown that restin inhibits the migration of endothelial cells in vitro but has no effect on the proliferation of these cells. A polyclonal antibody raised against endostatin cross-reacted with restin. Systemic administration of restin suppressed the growth of tumors in a xenograft renal carcinoma model.

Author List

Ramchandran R, Dhanabal M, Volk R, Waterman MJ, Segal M, Lu H, Knebelmann B, Sukhatme VP

Author

Ramani Ramchandran PhD Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Amino Acid Sequence
Antineoplastic Agents
Cell Division
Cell Line
Cell Movement
Cloning, Molecular
Collagen
Collagen Type XVIII
Endostatins
Humans
Intermediate Filament Proteins
Microtubule-Associated Proteins
Molecular Sequence Data
Neoplasm Proteins
Neoplasms, Experimental
Neovascularization, Physiologic
Peptide Fragments
Recombinant Proteins
Sequence Homology, Amino Acid
Transplantation, Heterologous
jenkins-FCD Prod-484 8aa07fc50b7f6d102f3dda2f4c7056ff84294d1d