Effect of metals on Candida albicans growth in the presence of chemical chelators and human abscess fluid. J Lab Clin Med 2001 Apr;137(4):284-9
Date
04/03/2001Pubmed ID
11283523DOI
10.1067/mlc.2001.113577Scopus ID
2-s2.0-0035068080 (requires institutional sign-in at Scopus site) 22 CitationsAbstract
Calprotectin is a calcium- and zinc-binding protein that is present in abscess fluid supernatants and appears to inhibit microbial growth through competition for zinc. In the present study, growth inhibition by chemical chelators was compared with that produced by human abscess fluid to determine whether other chelators, perhaps with different metal specificities, would have the same or different patterns of metal reversibility as abscess fluid. Zinc was found to be more potent than the other metals tested in reversing C. albicans growth inhibition by human abscess fluid and three chemical chelators, even though in some cases the stability constants of certain of these chelators were higher for other metals. For example, in the presence of the chelator diethylenetriaminopentaacetic acid, zinc stimulated Candida growth at a 10-fold lower concentration than did iron, even though this chelator has a stability constant for iron that is almost 10(10) higher than that for zinc. These results suggest that the zinc specificity of calprotectin's C. albicans growth inhibition can best be explained by the marked sensitivity of this organism to zinc deprivation rather than by selective binding of this metal by the protein.
Author List
Sohnle PG, Hahn BL, Karmarkar RAuthor
Peter G. Sohnle MS, MD Emeritus Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
CalciumCandida albicans
Chelating Agents
Empyema, Pleural
Humans
Zinc
