Fabrication of a bilayer scaffold for small diameter vascular applications. J Biomed Mater Res A 2018 Nov;106(11):2850-2862
Date
09/09/2018Pubmed ID
30194900DOI
10.1002/jbm.a.36473Scopus ID
2-s2.0-85052964955 (requires institutional sign-in at Scopus site) 21 CitationsAbstract
One of the greatest challenges plaguing cardiovascular tissue engineering has been the development of a compliant vascular graft. In this work, we report the development of a synthetic vascular graft with compliance similar to native arteries at physiological pressures. A bilayer scaffold was fabricated from a solid polymeric lumen made from poly(1,8 octanediol-co-citrate) (POC) and a microfibrous medial layer composed of type I collagen, elastin, and POC. Mechanical analysis revealed dynamic compliance, ~6.9% within 1% of native vessels, 5.9%. The burst pressure was an order of magnitude lower than native vessels (~400 mmHg vs. ~3000 mmHg) but was above physiological pressure ranges. Biocompatibility studies indicated the scaffold posed no acute cytotoxic risk to relevant cell types and supported the proliferation of vascular smooth muscle cells. In addition, upon exposure of the scaffold to whole blood, there was no statistically significant hemolysis, <2%. Overall this is a promising material system and scaffold to develop a biodegradable tissue-engineered vascular graft. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2850-2862, 2018.
Author List
Goins A, Ramaswamy V, Lichlyter D, Webb A, Allen JBAuthor
Josephine Allen PhD, MS Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AortaBiocompatible Materials
Blood Vessel Prosthesis
Cell Line
Cell Proliferation
Citrates
Collagen Type I
Elastin
Human Umbilical Vein Endothelial Cells
Humans
Materials Testing
Muscle, Smooth, Vascular
Myocytes, Smooth Muscle
Polymers
Tissue Engineering
Tissue Scaffolds
