Effect of high-salt diet on NO release and superoxide production in rat aorta. Am J Physiol Heart Circ Physiol 2004 Feb;286(2):H575-83
Date
10/07/2003Pubmed ID
14527935DOI
10.1152/ajpheart.00331.2003Scopus ID
2-s2.0-0942287336 (requires institutional sign-in at Scopus site) 102 CitationsAbstract
Sprague-Dawley rats were fed either a high-salt (HS) diet (4.0% NaCl) or a low-salt (LS) diet (0.4% NaCl) for 3 days. Nitric oxide (NO) and superoxide production were assessed in the thoracic aorta by evaluating the fluorescence signal intensity from 4,5-diaminofluorescein (DAF-2DA) and dihydroethidine, respectively. Methacholine caused increased NO release in the aortas from rats on a LS but not HS diet. The SOD mimetic tempol restored methacholine-induced NO release in aortas from rats on a HS diet. Methacholine also caused superoxide production in the aortas of rats on a HS diet but not in the aortas of rats on a LS diet. Tempol and N(G)-monomethyl-l-arginine eliminated methacholine-induced superoxide production in the aortas of rats on a HS diet. Aortic rings from rats on the HS diet showed impaired methacholine-induced relaxation, which was improved by tempol. Tempol alone caused a NO-dependent relaxation of norepinephrine-precontracted aortas that was significantly greater in the aortas of rats on the HS diet than in vessels from rats on the LS diet. These data suggest that a HS diet impairs endothelium-dependent relaxation via reduced NO levels and increased superoxide production.
Author List
Zhu J, Mori T, Huang T, Lombard JHMESH terms used to index this publication - Major topics in bold
AnimalsAorta
In Vitro Techniques
Male
Methacholine Chloride
Muscle, Smooth, Vascular
Nitric Oxide
Nitric Oxide Donors
Nitroso Compounds
Rats
Rats, Sprague-Dawley
Sodium Chloride, Dietary
Superoxides
Xanthine Oxidase
omega-N-Methylarginine