Faculty Collaboration Database

Production of MDC/CCL22 by human intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol 2001 Jun;280(6):G1217-26

Date

05/16/2001

Pubmed ID

11352815

DOI

10.1152/ajpgi.2001.280.6.G1217

Scopus ID

2-s2.0-0034972989 (requires institutional sign-in at Scopus site)   64 Citations

Abstract

The intestinal mucosa contains a subset of lymphocytes that produce Th2 cytokines, yet the signals responsible for the recruitment of these cells are poorly understood. Macrophage-derived chemokine (MDC/CCL22) is a recently described CC chemokine known to chemoattract the Th2 cytokine producing cells that express the receptor CCR4. The studies herein demonstrate the constitutive production of MDC/CCL22 in vivo by human colon epithelium and by epithelium of human intestinal xenografts. MDC/CCL22 mRNA expression and protein secretion was upregulated in colon epithelial cell lines in response to proinflammatory cytokines or infection with enteroinvasive bacteria. Inhibition of nuclear factor (NF)-kappaB activation abolished MDC/CCL22 expression in response to proinflammatory stimuli, demonstrating that MDC/CCL22 is a NF-kappaB target gene. In addition, tumor necrosis factor-alpha-induced MDC/CCL22 secretion was differentially modulated by Th1 and Th2 cytokines. Supernatants from the basal, but not apical, side of polarized epithelial cells induced a MDC/CCL22-dependent chemotaxis of CCR4-positive T cells. These studies demonstrate the constitutive and regulated production by intestinal epithelial cells of a chemokine known to function in the trafficking of T cells that produce anti-inflammatory cytokines.

Author List

Berin MC, Dwinell MB, Eckmann L, Kagnoff MF

Author

Michael B. Dwinell PhD Center Director, Professor in the Microbiology and Immunology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Cell Polarity
Chemokine CCL22
Chemokines, CC
Chemotaxis, Leukocyte
Colon
Cytokines
Escherichia coli Infections
Fetal Tissue Transplantation
Humans
Intestinal Mucosa
Intestines
Mice
Mice, SCID
NF-kappa B
RNA, Messenger
Salmonella Infections
T-Lymphocytes
Th2 Cells
Transplantation, Heterologous
Tumor Cells, Cultured