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An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast. J Cell Biol 2001 Apr 16;153(2):307-18

Date

04/20/2001

Pubmed ID

11309412

Pubmed Central ID

PMC2169461

DOI

10.1083/jcb.153.2.307

Scopus ID

2-s2.0-0035897422   79 Citations

Abstract

The localization of ASH1 mRNA to the distal tip of budding yeast cells is essential for the proper regulation of mating type switching in Saccharomyces cerevisiae. A localization element that is predominantly in the 3'-untranslated region (UTR) can direct this mRNA to the bud. Using this element in the three-hybrid in vivo RNA-binding assay, we identified a protein, Loc1p, that binds in vitro directly to the wild-type ASH1 3'-UTR RNA, but not to a mutant RNA incapable of localizing to the bud nor to several other mRNAs. LOC1 codes for a novel protein that recognizes double-stranded RNA structures and is required for efficient localization of ASH1 mRNA. Accordingly, Ash1p gets symmetrically distributed between daughter and mother cells in a loc1 strain. Surprisingly, Loc1p was found to be strictly nuclear, unlike other known RNA-binding proteins involved in mRNA localization which shuttle between the nucleus and the cytoplasm. We propose that efficient cytoplasmic ASH1 mRNA localization requires a previous interaction with specific nuclear factors.

Author List

Long RM, Gu W, Meng X, Gonsalvez G, Singer RH, Chartrand P

Author

Roy M. Long PhD Assistant Dean, Associate Professor in the Medical School Regional Campuses department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

3' Untranslated Regions
Amino Acid Sequence
Cell Nucleus
DNA-Binding Proteins
Fungal Proteins
Genes, Reporter
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Nuclear Proteins
Plasmids
Precipitin Tests
Protein Transport
RNA, Messenger
RNA-Binding Proteins
Recombinant Fusion Proteins
Regulatory Sequences, Nucleic Acid
Repressor Proteins
Reverse Transcriptase Polymerase Chain Reaction
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Transcription Factors
Two-Hybrid System Techniques