Upregulation of collagens detected by gene array in a model of flow-induced pulmonary vascular remodeling. Am J Physiol Heart Circ Physiol 2002 Feb;282(2):H414-22
Date
01/15/2002Pubmed ID
11788387DOI
10.1152/ajpheart.00292.2001Scopus ID
2-s2.0-0036084720 (requires institutional sign-in at Scopus site) 37 CitationsAbstract
We recently reported localized increased pulmonary arterial resistance, neointimal lesions, and medial thickening induced by aortopulmonary anastomosis in young pigs. This model was used to investigate changes in expression of genes potentially involved in pulmonary vascular remodeling employing a high throughput Atlas Human Cardiovascular Array carrying approximately 600 cardiovascular-related cDNA sequences. Data were confirmed by Northern analysis, Western blots, and histological examination. With the use of lower stringency conditions for hybridization, 56% of the 588 human genes on the array showed visible signal after autoradiography. Approximately 10% of the genes with visible hybridization were altered by shunt-induced high flow. Extracellular matrix and cell adhesion molecules were the most highly represented group of upregulated genes. To our knowledge, our data are the first to demonstrate flow-induced changes in gene expression using a combination of cross species cDNA arrays, homologous hybridization, immunospecific protein, and histology. Our observations expand the list of genes as putative candidates in pulmonary vascular remodeling and support the utility of cross-species microarray analysis in such applications.
Author List
Medhora M, Bousamra M 2nd, Zhu D, Somberg L, Jacobs ERMESH terms used to index this publication - Major topics in bold
AnimalsBase Sequence
Blotting, Northern
Collagen
Collagen Type I
Collagen Type III
Gene Expression
Hypertension, Pulmonary
Molecular Sequence Data
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis
Oligonucleotide Probes
Pulmonary Artery
Pulmonary Circulation
RNA, Messenger
Stress, Mechanical
Swine
Up-Regulation
