Faculty Collaboration Database

Histatin-1 Attenuates LPS-Induced Inflammatory Signaling in RAW264.7 Macrophages. Int J Mol Sci 2021 Jul 23;22(15)

Date

08/08/2021

Pubmed ID

34360629

Pubmed Central ID

PMC8345949

DOI

10.3390/ijms22157856

Scopus ID

2-s2.0-85110607836 (requires institutional sign-in at Scopus site)   53 Citations

Abstract

Macrophages play a critical role in the inflammatory response to environmental triggers, such as lipopolysaccharide (LPS). Inflammatory signaling through macrophages and the innate immune system are increasingly recognized as important contributors to multiple acute and chronic disease processes. Nitric oxide (NO) is a free radical that plays an important role in immune and inflammatory responses as an important intercellular messenger. In addition, NO has an important role in inflammatory responses in mucosal environments such as the ocular surface. Histatin peptides are well-established antimicrobial and wound healing agents. These peptides are important in multiple biological systems, playing roles in responses to the environment and immunomodulation. Given the importance of macrophages in responses to environmental triggers and pathogens, we investigated the effect of histatin-1 (Hst1) on LPS-induced inflammatory responses and the underlying molecular mechanisms in RAW264.7 (RAW) macrophages. LPS-induced inflammatory signaling, NO production and cytokine production in macrophages were tested in response to treatment with Hst1. Hst1 application significantly reduced LPS-induced NO production, inflammatory cytokine production, and inflammatory signaling through the JNK and NF-kB pathways in RAW cells. These results demonstrate that Hst1 can inhibit LPS-induced inflammatory mediator production and MAPK signaling pathways in macrophages.

Author List

Lee SM, Son KN, Shah D, Ali M, Balasubramaniam A, Shukla D, Aakalu VK

Author

Vinay Kumar Aakalu MPH, MD Chair, Professor in the Ophthalmology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Cytokines
Drug Evaluation, Preclinical
Histatins
Lipopolysaccharides
MAP Kinase Signaling System
Macrophage Activation
Macrophages
Mice
NF-kappa B
Nitric Oxide
RAW 264.7 Cells