Medical College of Wisconsin
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Murine Corneal Epithelial Wound Modeling. Methods Mol Biol 2021;2193:175-181

Date

08/19/2020

Pubmed ID

32808269

DOI

10.1007/978-1-0716-0845-6_17

Scopus ID

2-s2.0-85090240699 (requires institutional sign-in at Scopus site)   7 Citations

Abstract

A murine model of corneal epithelial wounding can be performed using simple injury and imaging methods. Here, we describe the creation of a central corneal epithelial defect using mechanical debridement under ophthalmic microscopic visualization. Subsequent monitoring with vital dye application and slit-lamp bio microscopy (slit-lamp) is described in detail.

Author List

Shah D, Aakalu VK

Author

Vinay Kumar Aakalu MPH, MD Chair, Professor in the Ophthalmology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Corneal Injuries
Debridement
Disease Models, Animal
Epithelium, Corneal
Mice
Mice, Inbred C57BL
Ophthalmologic Surgical Procedures
Wound Healing