Faculty Collaboration Database

Effects of histatin-1 peptide on human corneal epithelial cells. PLoS One 2017;12(5):e0178030

Date

05/26/2017

Pubmed ID

28542418

Pubmed Central ID

PMC5441629

DOI

10.1371/journal.pone.0178030

Scopus ID

2-s2.0-85019607000 (requires institutional sign-in at Scopus site)   35 Citations

Abstract

PURPOSE: Ocular surface and corneal epithelial wounds are common and potentially debilitating problems. Ideal treatments for these injuries would promote epithelial healing without inflammation, infection and scarring. In addition the best treatments would be cost-efficient, effective, non-toxic and easily applied. Histatin-1 peptides have been shown to be safe and effective enhancers of epithelial wound healing in other model systems. We sought to determine whether histatin-1 peptides could enhance human corneal epithelial wound healing in vitro.

METHODS: Histatin-1 peptides were applied to human corneal epithelial cells and compared over useful dose ranges in scratch assays using time-lapse microscopy. In addition, path finding analysis, cell spreading assays, toxicity and proliferation assays were performed to further characterize the effects of histatin-1 peptide on human corneal limbal epithelial (HCLE).

RESULTS: Histatin-1 enhanced human corneal epithelial wound healing in typical wound healing models. There was minimal toxicity and no significant enhancement of proliferation of corneal epithelium in response to histatin-1 application. Corneal epithelial spreading and pathfinding appeared to be enhanced by the application of histatin-1 peptides.

CONCLUSIONS: Histatin -1 peptide may enhance migration of HCLE cells and wound healing in vitro. These peptides may have benefit in corneal epithelial wounds and need to be investigated further.

Author List

Shah D, Ali M, Shukla D, Jain S, Aakalu VK

Author

Vinay Kumar Aakalu MPH, MD Chair, Professor in the Ophthalmology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Analysis of Variance
Bromodeoxyuridine
Cell Movement
Cell Proliferation
Cells, Cultured
Corneal Injuries
Dose-Response Relationship, Drug
Drug Evaluation, Preclinical
Epithelium, Corneal
Fluorescent Antibody Technique
Histatins
Humans
Protective Agents
Re-Epithelialization