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Human Lacrimal Gland Gene Expression. PLoS One 2017;12(1):e0169346

Date

01/13/2017

Pubmed ID

28081151

Pubmed Central ID

PMC5231359

DOI

10.1371/journal.pone.0169346

Scopus ID

2-s2.0-85009383195 (requires institutional sign-in at Scopus site)   15 Citations

Abstract

BACKGROUND: The study of human lacrimal gland biology and development is limited. Lacrimal gland tissue is damaged or poorly functional in a number of disease states including dry eye disease. Development of cell based therapies for lacrimal gland diseases requires a better understanding of the gene expression and signaling pathways in lacrimal gland. Differential gene expression analysis between lacrimal gland and other embryologically similar tissues may be helpful in furthering our understanding of lacrimal gland development.

METHODS: We performed global gene expression analysis of human lacrimal gland tissue using Affymetrix ® gene expression arrays. Primary data from our laboratory was compared with datasets available in the NLM GEO database for other surface ectodermal tissues including salivary gland, skin, conjunctiva and corneal epithelium.

RESULTS: The analysis revealed statistically significant difference in the gene expression of lacrimal gland tissue compared to other ectodermal tissues. The lacrimal gland specific, cell surface secretory protein encoding genes and critical signaling pathways which distinguish lacrimal gland from other ectodermal tissues are described.

CONCLUSIONS: Differential gene expression in human lacrimal gland compared with other ectodermal tissue types revealed interesting patterns which may serve as the basis for future studies in directed differentiation among other areas.

Author List

Aakalu VK, Parameswaran S, Maienschein-Cline M, Bahroos N, Shah D, Ali M, Krishnakumar S

Author

Vinay Kumar Aakalu MPH, MD Chair, Professor in the Ophthalmology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Databases, Genetic
Dry Eye Syndromes
Eye Proteins
Female
Gene Expression Regulation
Humans
Lacrimal Apparatus
Male