Faculty Collaboration Database

Histatin-1 Expression in Human Lacrimal Epithelium. PLoS One 2016;11(1):e0148018

Date

01/30/2016

Pubmed ID

26824896

Pubmed Central ID

PMC4732786

DOI

10.1371/journal.pone.0148018

Scopus ID

2-s2.0-84958225616 (requires institutional sign-in at Scopus site)   22 Citations

Abstract

BACKGROUND: Study of human lacrimal cell biology is limited by poor access to tissue samples, heterogeneous cell composition of tissue and a lack of established lacrimal epithelial markers. In order to further our understanding of lacrimal cell biology, we sought to find a better marker for human lacrimal epithelial cells, compared to what has been reported in the literature.

METHODS: We utilized human Muller's muscle conjunctival resection (MMCR) specimens containing accessory lacrimal gland (ALG) and cadaveric main lacrimal gland (MLG) as sources of lacrimal tissue. Candidate markers were sought using human ALG tissue from MMCR specimens, isolated by laser capture microdissection (LCM). Affymetrix® analysis was performed on total RNA isolated from FFPE samples to profile transcription in ALG. MMCR tissue sections were assessed by immunofluorescence using antibodies for histatin-1, lactoferrin, E-cadherin (E-cad) and alpha-smooth muscle actin (ASMA). Reverse transcriptase polymerase chain reaction (RT-PCR) analysis was performed to analyze the expression of histatin-1, E-cad and lactoferrin from cadaveric MLG.

RESULTS: Histatin-1 is expressed in ALG and MLG, localizes to lacrimal epithelium, and to a greater degree than do other putative lacrimal epithelial markers.

CONCLUSIONS: Histatin-1 is a good marker for human lacrimal epithelium in ALG and MLG and can be used to identify lacrimal cells in future studies.

Author List

Shah D, Ali M, Pasha Z, Jaboori AJ, Jassim SH, Jain S, Aakalu VK

Author

Vinay Kumar Aakalu MPH, MD Chair, Professor in the Ophthalmology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Actins
Biomarkers
Cadherins
Epithelial Cells
Epithelium
Formaldehyde
Gene Expression
Gene Expression Profiling
Histatins
Humans
Lacrimal Apparatus
Lactoferrin
Laser Capture Microdissection
Oligonucleotide Array Sequence Analysis
RNA, Messenger
Tissue Fixation