Faculty Collaboration Database

Microbiota-derived metabolite promotes HDAC3 activity in the gut. Nature 2020 Oct;586(7827):108-112

Date

07/31/2020

Pubmed ID

32731255

Pubmed Central ID

PMC7529926

DOI

10.1038/s41586-020-2604-2

Scopus ID

2-s2.0-85088807655 (requires institutional sign-in at Scopus site)   217 Citations

Abstract

The coevolution of mammalian hosts and their beneficial commensal microbes has led to development of symbiotic host-microbiota relationships¹. Epigenetic machinery permits mammalian cells to integrate environmental signals²; however, how these pathways are fine-tuned by diverse cues from commensal bacteria is not well understood. Here we reveal a highly selective pathway through which microbiota-derived inositol phosphate regulates histone deacetylase 3 (HDAC3) activity in the intestine. Despite the abundant presence of HDAC inhibitors such as butyrate in the intestine, we found that HDAC3 activity was sharply increased in intestinal epithelial cells of microbiota-replete mice compared with germ-free mice. This divergence was reconciled by the finding that commensal bacteria, including Escherichia coli, stimulated HDAC activity through metabolism of phytate and production of inositol-1,4,5-trisphosphate (InsP₃). Both intestinal exposure to InsP₃ and phytate ingestion promoted recovery following intestinal damage. Of note, InsP₃ also induced growth of intestinal organoids derived from human tissue, stimulated HDAC3-dependent proliferation and countered butyrate inhibition of colonic growth. Collectively, these results show that InsP₃ is a microbiota-derived metabolite that activates a mammalian histone deacetylase to promote epithelial repair. Thus, HDAC3 represents a convergent epigenetic sensor of distinct metabolites that calibrates host responses to diverse microbial signals.

Author List

Wu SE, Hashimoto-Hill S, Woo V, Eshleman EM, Whitt J, Engleman L, Karns R, Denson LA, Haslam DB, Alenghat T

Author

Emily M. Eshleman PhD Assistant Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Gastrointestinal Microbiome
Histone Deacetylases
Humans
Inositol 1,4,5-Trisphosphate
Intestinal Mucosa
Intestines
Mice
Mice, Inbred C57BL
Organoids
Phytic Acid
Symbiosis