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Agonist-dependent generation of lipoxins from rat basophilic leukemia cell (RBL-1). Biochim Biophys Acta 1989 Aug 22;1004(3):332-6

Date

08/22/1989

Pubmed ID

2503031

DOI

10.1016/0005-2760(89)90081-7

Scopus ID

2-s2.0-0024441831   5 Citations

Abstract

Incubation of RBL-1 cells in the presence of 15-HPETE and various agonists generated lipoxins and several isomers. Addition of either A23187, fMLP or PMA modulated the number of isomers and amount of lipoxins produced. Administration of A23187 yielded the largest amount of product (5.3 +/- 1.6 micrograms per 10(8) cells) and generated a total of six and three isomers of LXA4 and B4, respectively. This was 2-fold greater than fMLP, which produced a total of two isomers of LXA4 and LXB4. Addition of PMA generated only LXA4 (0.68 +/- 0.26 micrograms). This is similar to the control receiving only 15-HPETE. Biologically derived LXA4 (3 nM) isolated from RBL-1 incubations contracted a rat tail artery preparation to 12% of the maximum induced by phenylephrine (0.125 microM), whereas LXA4 standard (3 nM) elicited 17.6% of the maximum contraction. These results indicate that RBL-1 cells can utilize exogenous 15-HPETE to generate biologically active lipoxins. Further, the yield and isomers of lipoxins can be modified by different agonists.

Author List

Ng CF, Lam BK, Pritchard KA Jr, Stemerman MB, Hejny P, Wong PY

Author

Kirkwood A. Pritchard PhD Professor in the Surgery department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Calcimycin
Hydroxyeicosatetraenoic Acids
Leukotrienes
Lipid Peroxides
Lipoxins
N-Formylmethionine Leucyl-Phenylalanine
Rats
Tetradecanoylphorbol Acetate
Tumor Cells, Cultured
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a