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The ligninase of Phanerochaete chrysosporium generates cation radicals from methoxybenzenes. J Biol Chem 1985 Mar 10;260(5):2609-12

Date

03/10/1985

Pubmed ID

2982828

Scopus ID

2-s2.0-0021910164 (requires institutional sign-in at Scopus site)   293 Citations

Abstract

The hemoprotein ligninase of Phanerochaete chrysosporium catalyzes, in the presence of H2O2, a variety of seemingly different oxidations in lignin and lignin model compounds. Here we show that the enzyme also catalyzes the oxidation of various methoxybenzenes. ESR spectroscopy shows that the compounds are oxidized to aryl cation radicals. These decompose, evidently by H2O addition. Thus, 1,4-dimethoxybenzene is converted to p-benzoquinone and methanol. We propose a unified mechanism, based on formation of aryl cation radicals, to explain the various reactions catalyzed by the ligninase.

Author List

Kersten PJ, Tien M, Kalyanaraman B, Kirk TK

Author

Balaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Anisoles
Basidiomycota
Electron Spin Resonance Spectroscopy
Free Radicals
Hydrogen Peroxide
Methanol
Oxygenases
Spectrophotometry, Ultraviolet
Time Factors