Combined developmental toxicity of bisphenol A and genistein in micromass cultures of rat embryonic limb bud and midbrain cells. Toxicol In Vitro 2011 Feb;25(1):153-9
Date
11/03/2010Pubmed ID
21034807DOI
10.1016/j.tiv.2010.10.010Scopus ID
2-s2.0-78650520794 (requires institutional sign-in at Scopus site) 11 CitationsAbstract
Bisphenol A (BPA), widely used in industry and dentistry, and genistein (GEN), the predominant component of soy product, are both known environmental estrogen. In the present study, we investigated the developmental toxicity of BPA and GEN and their combined effect using micromass test, which is one of three standard alternative developmental toxicity tests recommended by European Center for the Validation of Alternative Methods (ECVAM). The results showed that IC50-P (cell proliferation) and IC50-D (cell differentiation) of BPA and GEN were approximately 20 and 5 μg/ml, respectively. No observed adverse effect level (NOAEL) of BPA and GEN were 10 and 0.94 μg/ml, respectively. The manifestation of BPA as a teratogen was insufficient, although the "low dose" effect should be paid attention to. While the evidence of GEN as a teratogen was solid, especially with the consideration of "high dose" application in clinical treatment. The combined effect of BPA and GEN was generally additive action except that in MB proliferation.
Author List
Xiao Y, Liu R, Xing L, Xu Y, Shang L, Hao WAuthor
Yang Xiao Assistant Professor in the Physiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBenzhydryl Compounds
Cell Differentiation
Cell Proliferation
Cells, Cultured
Drug Interactions
Embryo, Mammalian
Environmental Pollutants
Estrogens, Non-Steroidal
Genistein
Limb Buds
Mesencephalon
Neurons
No-Observed-Adverse-Effect Level
Osmolar Concentration
Phenols
Phytoestrogens
Rats
Rats, Sprague-Dawley
Teratogens
Toxicity Tests
