Structural and biomechanical characterizations of porcine myocardial extracellular matrix. J Mater Sci Mater Med 2012 Aug;23(8):1835-47
Date
05/16/2012Pubmed ID
22584822Pubmed Central ID
PMC3523096DOI
10.1007/s10856-012-4660-0Scopus ID
2-s2.0-84864915724 (requires institutional sign-in at Scopus site) 67 CitationsAbstract
Extracellular matrix (ECM) of myocardium plays an important role to maintain a multilayered helical architecture of cardiomyocytes. In this study, we have characterized the structural and biomechanical properties of porcine myocardial ECM. Fresh myocardium were decellularized in a rotating bioreactor using 0.1 % sodium dodecyl sulfate solution. Masson's trichrome staining and SEM demonstrated the removal of cells and preservation of the interconnected 3D cardiomyocyte lacunae. Movat's pentachrome staining showed the preservation of cardiac elastin ultrastructure and vascular elastin distribution/alignment. DNA assay result confirmed a 98.59 % reduction in DNA content; the acellular myocardial scaffolds were found completely lack of staining for the porcine α-Gal antigen; and the accelerating enzymatic degradation assessment showed a constant degradation rate. Tensile and shear properties of the acellular myocardial scaffolds were also evaluated. Our observations showed that the acellular myocardial ECM possessed important traits of biodegradable scaffolds, indicating the potentials in cardiac regeneration and whole heart tissue engineering.
Author List
Wang B, Tedder ME, Perez CE, Wang G, de Jongh Curry AL, To F, Elder SH, Williams LN, Simionescu DT, Liao JAuthor
Bo Wang PhD Assistant Professor in the Biomedical Engineering department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCell-Free System
Extracellular Matrix
Materials Testing
Myocardium
Shear Strength
Swine
Tensile Strength
Tissue Engineering
Tissue Scaffolds