Boronate probes as diagnostic tools for real time monitoring of peroxynitrite and hydroperoxides. Chem Res Toxicol 2012 Sep 17;25(9):1793-9
Date
06/27/2012Pubmed ID
22731669Pubmed Central ID
PMC3501381DOI
10.1021/tx300164jScopus ID
2-s2.0-84866386085 (requires institutional sign-in at Scopus site) 197 CitationsAbstract
Boronates, a group of organic compounds, are emerging as one of the most effective probes for detecting and quantifying peroxynitrite, hypochlorous acid, and hydrogen peroxide. Boronates react with peroxynitrite nearly a million times faster than with hydrogen peroxide. Boronate-containing fluorogenic compounds have been used to monitor real time generation of peroxynitrite in cells and for imaging hydrogen peroxide in living animals. This perspective highlights potential applications of boronates and other fluorescent probes to high-throughput analyses of peroxynitrite and hydroperoxides in toxicological studies.
Author List
Zielonka J, Sikora A, Hardy M, Joseph J, Dranka BP, Kalyanaraman BAuthors
Micael Joel Hardy PhD Visiting Assistant Professor in the Biophysics department at Medical College of WisconsinBalaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of Wisconsin
Jacek M. Zielonka PhD Assistant Professor in the Biophysics department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsBoronic Acids
Cell Line
Chromatography, High Pressure Liquid
Fluorescent Dyes
Hydrogen Peroxide
Hypochlorous Acid
Kinetics
Macrophages
Mass Spectrometry
Mice
Oxidation-Reduction
Peroxynitrous Acid