DNA expression analysis: serial analysis of gene expression, microarrays and kidney disease. Curr Opin Nephrol Hypertens 2003 Jul;12(4):407-14
Date
06/20/2003Pubmed ID
12815337DOI
10.1097/00041552-200307000-00009Scopus ID
2-s2.0-0037765263 (requires institutional sign-in at Scopus site) 15 CitationsAbstract
PURPOSE OF REVIEW: Expression profiling using serial analysis of gene expression and microarray technologies allows global description of expressed genes present in biological systems. Although relatively new technologies, each having been developed in the mid-1990s, both have become established and widely used tools for identification of gene networks and gene function.
RECENT FINDINGS: This review highlights DNA expression analyses published in 2002, emphasizing primarily serial analysis of gene expression and microarray technologies. We focus on the applicability of DNA expression analysis to renal disease, especially as some investigators have developed custom serial analysis of gene expression kidney libraries and kidney disease-specific 'designer chip' microarrays. Data analysis techniques and statistics are also discussed, since the challenge is generation of accurate messenger RNA profiles and interpretation of data in a manner that is both coherent and reproducible.
SUMMARY: Because kidney disease pathophysiology is complex, expression analysis can identify candidate nephropathy pathogenesis genes and gene networks, which eventually could become targets for therapeutic intervention.
Author List
Hayden PS, El-Meanawy A, Schelling JR, Sedor JRAuthor
Ashraf El-Meanawy MD, PhD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Base SequenceDNA
Gene Expression Profiling
Gene Expression Regulation
Gene Library
Humans
Kidney Diseases
Oligonucleotide Array Sequence Analysis
Sequence Analysis, DNA
