Unprecedented hydroxyl radical-dependent two-step chemiluminescence production by polyhalogenated quinoid carcinogens and H2O2. Proc Natl Acad Sci U S A 2012 Oct 02;109(40):16046-51
Date
09/19/2012Pubmed ID
22988069Pubmed Central ID
PMC3479549DOI
10.1073/pnas.1204479109Scopus ID
2-s2.0-84867036556 (requires institutional sign-in at Scopus site) 95 CitationsAbstract
Most chemiluminescence (CL) reactions usually generate only one-step CL, which is rarely dependent on the highly reactive and biologically/environmentally important hydroxyl radicals ((•)OH). Here, we show that an unprecedented two-step CL can be produced by the carcinogenic tetrachloro-1,4-benzoquinone (also known as p-chloranil) and H(2)O(2), which was found to be well-correlated to and directly dependent on its two-step metal-independent production of (•)OH. We proposed that (•)OH-dependent formation of quinone-dioxetane and electronically excited carbonyl species might be responsible for this unusual two-step CL production by tetrachloro-1,4-benzoquinone/H(2)O(2). This is a unique report of a previously undefined two-step CL-producing system that is dependent on intrinsically formed (•)OH. These findings may have potential applications in detecting and quantifying (•)OH and the ubiquitous polyhalogenated aromatic carcinogens, which may have broad biological and environmental implications for future research on these types of important species.
Author List
Zhu BZ, Mao L, Huang CH, Qin H, Fan RM, Kalyanaraman B, Zhu JGAuthor
Balaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
CarcinogensChloranil
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Fluorescence
Gas Chromatography-Mass Spectrometry
Hydrogen Peroxide
Hydroxyl Radical
Luminescence
Models, Chemical
Molecular Structure
