Faculty Collaboration Database

Normal human pluripotent stem cell lines exhibit pervasive mosaic aneuploidy. PLoS One 2011;6(8):e23018

Date

08/23/2011

Pubmed ID

21857983

Pubmed Central ID

PMC3156708

DOI

10.1371/journal.pone.0023018

Scopus ID

2-s2.0-80051674461 (requires institutional sign-in at Scopus site)   53 Citations

Abstract

Human pluripotent stem cell (hPSC) lines have been considered to be homogeneously euploid. Here we report that normal hPSC--including induced pluripotent--lines are karyotypic mosaics of euploid cells intermixed with many cells showing non-clonal aneuploidies as identified by chromosome counting, spectral karyotyping (SKY) and fluorescent in situ hybridization (FISH) of interphase/non-mitotic cells. This mosaic aneuploidy resembles that observed in progenitor cells of the developing brain and preimplantation embryos, suggesting that it is a normal, rather than pathological, feature of stem cell lines. The karyotypic heterogeneity generated by mosaic aneuploidy may contribute to the reported functional and phenotypic heterogeneity of hPSCs lines, as well as their therapeutic efficacy and safety following transplantation.

Author List

Peterson SE, Westra JW, Rehen SK, Young H, Bushman DM, Paczkowski CM, Yung YC, Lynch CL, Tran HT, Nickey KS, Wang YC, Laurent LC, Loring JF, Carpenter MK, Chun J

MESH terms used to index this publication - Major topics in bold

Aneuploidy
Animals
Cell Culture Techniques
Cell Line
Female
Humans
In Situ Hybridization, Fluorescence
Karyotyping
Mice
Pluripotent Stem Cells
Spectral Karyotyping