Normal human pluripotent stem cell lines exhibit pervasive mosaic aneuploidy. PLoS One 2011;6(8):e23018
Date
08/23/2011Pubmed ID
21857983Pubmed Central ID
PMC3156708DOI
10.1371/journal.pone.0023018Scopus ID
2-s2.0-80051674461 (requires institutional sign-in at Scopus site) 53 CitationsAbstract
Human pluripotent stem cell (hPSC) lines have been considered to be homogeneously euploid. Here we report that normal hPSC--including induced pluripotent--lines are karyotypic mosaics of euploid cells intermixed with many cells showing non-clonal aneuploidies as identified by chromosome counting, spectral karyotyping (SKY) and fluorescent in situ hybridization (FISH) of interphase/non-mitotic cells. This mosaic aneuploidy resembles that observed in progenitor cells of the developing brain and preimplantation embryos, suggesting that it is a normal, rather than pathological, feature of stem cell lines. The karyotypic heterogeneity generated by mosaic aneuploidy may contribute to the reported functional and phenotypic heterogeneity of hPSCs lines, as well as their therapeutic efficacy and safety following transplantation.
Author List
Peterson SE, Westra JW, Rehen SK, Young H, Bushman DM, Paczkowski CM, Yung YC, Lynch CL, Tran HT, Nickey KS, Wang YC, Laurent LC, Loring JF, Carpenter MK, Chun JMESH terms used to index this publication - Major topics in bold
AneuploidyAnimals
Cell Culture Techniques
Cell Line
Female
Humans
In Situ Hybridization, Fluorescence
Karyotyping
Mice
Pluripotent Stem Cells
Spectral Karyotyping