COPI transport complexes bind to specific RNAs in neuronal cells. Hum Mol Genet 2013 Feb 15;22(4):729-36
Date
11/24/2012Pubmed ID
23175440Pubmed Central ID
PMC3988478DOI
10.1093/hmg/dds480Scopus ID
2-s2.0-84873034506 (requires institutional sign-in at Scopus site) 40 CitationsAbstract
Our fundamental understanding of how several thousand diverse RNAs are recognized in the soma, sorted, packaged, transported and localized within the cell is fragmentary. The COPa and COPb proteins of the coatomer protein I (COPI) vesicle complex were reported to interact with specific RNAs and represent a candidate RNA sorting and transport system. To determine the RNA-binding profile of Golgi-derived COPI in neuronal cells, we performed formaldehyde-linked RNA immunoprecipitation, followed by high-throughput sequencing, a process we term FLRIP-Seq (FLRIP, formaldehyde-cross-linked immunoprecipitation). We demonstrate that COPa co-immunoprecipitates a specific set of RNAs that are enriched in G-quadruplex motifs and fragile X mental retardation protein-associated RNAs and that encode factors that predominantly localize to the plasma membrane and cytoskeleton and function within signaling pathways. These data support the novel function of COPI in inter-compartmental trafficking of RNA.
Author List
Todd AG, Lin H, Ebert AD, Liu Y, Androphy EJAuthor
Allison D. Ebert PhD Associate Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
3' Untranslated RegionsAnimals
Antigens, Neoplasm
Base Sequence
Cell Line, Tumor
Cell Membrane
Coat Protein Complex I
Coatomer Protein
Consensus Sequence
Cytoskeleton
Fragile X Mental Retardation Protein
Mice
Nerve Tissue Proteins
Neurites
Protein Binding
Protein Subunits
Protein Transport
RNA Transport
RNA, Messenger
RNA-Binding Proteins
Sequence Analysis, RNA
Transcriptome
