Follicle selection in cyclic guinea pigs with active immunization against inhibin alpha-subunit. Life Sci 2000;66(25):2489-97
Date
07/14/2000Pubmed ID
10894091DOI
10.1016/s0024-3205(00)80008-9Scopus ID
2-s2.0-17044445386 (requires institutional sign-in at Scopus site) 6 CitationsAbstract
Experiments were conducted to elucidate the mechanisms of active immunization against inhibin on ovarian follicular development and selection in guinea pigs. Estrous cycle was synchronized in experimental guinea pigs by implanting progesterone containing tubes. Antibodies that bound 125I-labeled bovine inhibin were produced by all guinea pigs receiving the inhibin vaccine (recombinant ovine alpha-subunit in oil emulsion) without any effects on duration of the estrous cycle. Active immunization against inhibin increased the plasma concentrations of progesterone during the luteal phase and the plasma concentrations of estradiol but failed to increase the plasma concentration of follicle-stimulating hormone (FSH) during preovulatory period. The treatment also increased the number of corpora lutea (from 1.3+/-0.3 to 7.0+/-1.6 per each ovary), and preovulatory sized follicles (from 1.8+/-0.6 to 7.0+/-1.6 per each ovary), and follicles stained positively for inhibin alpha-subunit (from 2.3+/-0.5 to 6.3+/-1.3 per each ovary) significantly. The results indicate that active immunization against inhibin enhances ovulation rate by affecting the follicle selection and only dominant follicle can be stained for inhibin alpha-subunit in guinea pigs. This study is firstly to provide direct evidence that inhibins play important role in follicle selections in guinea pigs.
Author List
Shi F, Mochida K, Ogura A, Matsuda J, Suzuki O, Watanabe G, Hutz RJ, Tsonis CG, Suzuki AK, Taya KAuthor
Reinhold J. Hutz in the Biological Sciences department at University of Wisconsin - MilwaukeeMESH terms used to index this publication - Major topics in bold
AnimalsAntibodies
Estradiol
Female
Follicle Stimulating Hormone
Guinea Pigs
Immunohistochemistry
Inhibins
Ovarian Follicle
Ovulation
Peptides
Progesterone
Vaccination
Vaccines, Synthetic
