Faculty Collaboration Database

Secretion of ovarian inhibin and its physiologic roles in the regulation of follicle-stimulating hormone secretion during the estrous cycle of the female guinea pig. Biol Reprod 1999 Jan;60(1):78-84

Date

12/22/1998

Pubmed ID

9858489

DOI

10.1095/biolreprod60.1.78

Scopus ID

2-s2.0-0032919706 (requires institutional sign-in at Scopus site)   47 Citations

Abstract

To characterize inhibin secretion during the estrous cycle in guinea pigs, the concentrations of plasma inhibin, estradiol, progesterone, and FSH were determined. A significant positive correlation was observed between inhibin and estradiol throughout the estrous cycle. Plasma inhibin and estradiol started to increase a few days before ovulation (Day 0 = day of estimated ovulation), and decreased after ovulation. These two hormones remained low during the luteal phase. The immunoreactivity of inhibin alpha, betaA, and betaB subunits was colocalized in the granulosa cells of one or two healthy large follicles in the ovary before ovulation. There was no positive reaction of inhibin alpha and beta subunits in the corpora lutea or other follicles. Ovariectomy resulted in an abrupt decrease in plasma inhibin and a significant increase in plasma FSH. Injection of anti-inhibin serum into adult female guinea pigs induced an elevation in plasma FSH in a dose-dependent manner. This report presents the first description of sequential changes in plasma inhibin and estradiol during the estrous cycle of guinea pigs. Results suggest that inhibin is secreted mainly by granulosa cells of a few healthy large follicles in the ovary and that it plays an important role in the regulation of FSH secretion during the estrous cycle in guinea pigs.

Author List

Shi F, Ozawa M, Komura H, Yang P, Trewin AL, Hutz RJ, Watanabe G, Taya K

Author

Reinhold J. Hutz in the Biological Sciences department at University of Wisconsin - Milwaukee

MESH terms used to index this publication - Major topics in bold

Animals
Corpus Luteum
Estradiol
Estrus
Female
Follicle Stimulating Hormone
Granulosa Cells
Guinea Pigs
Homeostasis
Immunization, Passive
Immunohistochemistry
Inhibins
Ovarian Follicle
Ovariectomy
Ovary
Ovulation
Progesterone
Radioimmunoassay