The use of vital dyes to assess embryonic viability in the hamster, Mesocricetus auratus. Stain Technol 1985 May;60(3):163-7
Date
05/01/1985Pubmed ID
2411021DOI
10.3109/10520298509113907Scopus ID
2-s2.0-84907131275 (requires institutional sign-in at Scopus site) 19 CitationsAbstract
Experiments were designed to assess the use of the vital dyes trypan blue and fluorescein diacetate as indicators of the viability of hamster ova and embryos. Exclusion of trypan blue and fluorescence with fluorescein diacetate showed high correlations with uptake of [3H]uridine by ova and further development of embryos in vitro. Ova killed by freezing and thawing incorporated [3H]uridine at background levels. Trypan blue exclusion and fluorescein diacetate uptake were highly correlated with each other (r = 0.99). Trypan blue and fluorescein diacetate serve as excellent indices of viability in ova and early embryos of hamsters.
Author List
Hutz RJ, DeMayo FJ, Dukelow WRAuthor
Reinhold J. Hutz in the Biological Sciences department at University of Wisconsin - MilwaukeeMESH terms used to index this publication - Major topics in bold
AnimalsCell Survival
Cricetinae
Embryo, Mammalian
Female
Fluoresceins
Mesocricetus
Ovum
Staining and Labeling
Tritium
Trypan Blue
Uridine
