Flavorubredoxin, an inducible catalyst for nitric oxide reduction and detoxification in Escherichia coli. J Biol Chem 2002 Mar 08;277(10):8172-7
Date
12/26/2001Pubmed ID
11751865DOI
10.1074/jbc.M110471200Scopus ID
2-s2.0-0037040921 (requires institutional sign-in at Scopus site) 282 CitationsAbstract
Nitric oxide (NO) is a poison, and organisms employ diverse systems to protect against its harmful effects. In Escherichia coli, ygaA encodes a transcription regulator (b2709) controlling anaerobic NO reduction and detoxification. Adjacent to ygaA and oppositely transcribed are ygaK (encoding a flavorubredoxin (flavoRb) (b2710) with a NO-binding non-heme diiron center) and ygbD (encoding a NADH:(flavo)Rb oxidoreductase (b2711)), which function in NO reduction and detoxification. Mutation of either ygaA or ygaK eliminated inducible anaerobic NO metabolism, whereas ygbD disruption partly impaired the activity. NO-sensitive [4Fe-4S] (de)hydratases, including the Krebs cycle aconitase and the Entner-Doudoroff pathway 6-phosphogluconate dehydratase, were more susceptible to inactivation in ygaK or ygaA mutants than in the parental strain, and these metabolic poisonings were associated with conditional growth inhibitions. flavoRb (NO reductase) and flavohemoglobin (NO dioxygenase) maximally metabolized and detoxified NO in anaerobic and aerobic E. coli, respectively, whereas both enzymes scavenged NO under microaerobic conditions. We suggest designation of the ygaA-ygaK-ygbD gene cluster as the norRVW modulon for NO reduction and detoxification.
Author List
Gardner AM, Helmick RA, Gardner PRAuthor
Ryan Helmick MD Associate Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Aconitate HydrataseChromosomes
DNA Transposable Elements
Dose-Response Relationship, Drug
Escherichia coli
Escherichia coli Proteins
Hydro-Lyases
Models, Genetic
Models, Molecular
Multigene Family
Mutagenesis
Mutation
Nitric Oxide
Operon
Plasmids
Protein Binding
Time Factors
Transcription Factors
Transcription, Genetic
