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Mirror-symmetric microtubule assembly and cell interactions drive lumen formation in the zebrafish neural rod. EMBO J 2013 Jan 09;32(1):30-44

Date

12/04/2012

Pubmed ID

23202854

Pubmed Central ID

PMC3545300

DOI

10.1038/emboj.2012.305

Scopus ID

2-s2.0-84872861885   39 Citations

Abstract

By analysing the cellular and subcellular events that occur in the centre of the developing zebrafish neural rod, we have uncovered a novel mechanism of cell polarisation during lumen formation. Cells from each side of the neural rod interdigitate across the tissue midline. This is necessary for localisation of apical junctional proteins to the region where cells intersect the tissue midline. Cells assemble a mirror-symmetric microtubule cytoskeleton around the tissue midline, which is necessary for the trafficking of proteins required for normal lumen formation, such as partitioning defective 3 and Rab11a to this point. This occurs in advance and is independent of the midline cell division that has been shown to have a powerful role in lumen organisation. To our knowledge, this is the first example of the initiation of apical polarisation part way along the length of a cell, rather than at a cell extremity. Although the midline division is not necessary for apical polarisation, it confers a morphogenetic advantage by efficiently eliminating cellular processes that would otherwise bridge the developing lumen.

Author List

Buckley CE, Ren X, Ward LC, Girdler GC, Araya C, Green MJ, Clark BS, Link BA, Clarke JD

Author

Brian A. Link PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Body Patterning
Carrier Proteins
Cell Communication
Cell Division
Cell Movement
Cell Polarity
Embryo, Nonmammalian
Green Fluorescent Proteins
Luminescent Agents
Microtubules
Mutation
Neural Tube
Neurulation
Nocodazole
Protein Transport
Recombinant Fusion Proteins
Tubulin Modulators
Zebrafish
Zebrafish Proteins
rab GTP-Binding Proteins
jenkins-FCD Prod-486 e3098984f26de787f5ecab75090d0a28e7f4f7c0