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Stat5 is essential for early B cell development but not for B cell maturation and function. J Immunol 2007 Jul 15;179(2):1068-79

Date

07/10/2007

Pubmed ID

17617599

DOI

10.4049/jimmunol.179.2.1068

Scopus ID

2-s2.0-34548807675   44 Citations

Abstract

The two closely related Stat5 (Stat5A and Stat5B) proteins are activated by a broad spectrum of cytokines. However, with the complication of the involvement of Stat5A/5B in stem cell function, the role of Stat5A/5B in the development and function of lymphocytes, especially B cells, is not fully understood. In this study, we demonstrated that Stat5A/5B(-/-) fetal liver cells had severe diminution of B cell progenitors but clearly had myeloid progenitors. Consistently, the mutant fetal liver cells could give rise to hemopoietic progenitors and myeloid cells but not B cells beyond pro-B cell progenitors in lethally irradiated wild-type or Jak3(-/-) mice. Deletion of Stat5A/5B in vitro directly impaired IL-7-mediated B cell expansion. Of note, reintroduction of Stat5A back into Stat5A/5B(-/-) fetal liver cells restored their abilities to develop B cells. Importantly, CD19-Cre-mediated deletion of Stat5A/5B in the B cell compartment specifically impaired early B cell development but not late B cell maturation. Moreover, the B cell-specific deletion of Stat5A/5B did not impair splenic B cell survival, proliferation, and Ig production. Taken together, these data demonstrate that Stat5A/5B directly control IL-7-mediated early B cell development but are not required for B cell maturation and Ig production.

Author List

Dai X, Chen Y, Di L, Podd A, Li G, Bunting KD, Hennighausen L, Wen R, Wang D

Author

Demin Wang PhD Assistant Professor in the Microbiology and Immunology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Antibody Formation
B-Lymphocytes
Blotting, Western
Cell Differentiation
Flow Cytometry
In Situ Nick-End Labeling
Interleukin-7
Liver
Mice
Mice, Mutant Strains
Myeloid Progenitor Cells
Reverse Transcriptase Polymerase Chain Reaction
STAT5 Transcription Factor
jenkins-FCD Prod-469 c3fc8ab87196149f9b23743c01b947d47e7319e5