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Phospholipase Cgamma2 contributes to light-chain gene activation and receptor editing. Mol Cell Biol 2007 Sep;27(17):5957-67

Date

06/27/2007

Pubmed ID

17591700

Pubmed Central ID

PMC1952164

DOI

10.1128/MCB.02273-06

Scopus ID

2-s2.0-34548203816   17 Citations

Abstract

Phospholipase Cgamma2 (PLCgamma2) is critical for pre-B-cell receptor (pre-BCR) and BCR signaling. Current studies discovered that PLCgamma2-deficient mice had reduced immunoglobulin lambda (Iglambda) light-chain usage throughout B-cell maturation stages, including transitional type 1 (T1), transitional type 2 (T2), and mature follicular B cells. The reduction of Iglambda rearrangement by PLCgamma2 deficiency was not due to specifically increased apoptosis or decreased proliferation of mutant Iglambda+ B cells, as lack of PLCgamma2 exerted a similar effect on apoptosis and proliferation of both Iglambda+ and Igkappa+ B cells. Moreover, PLCgamma2-deficient IgHEL transgenic B cells exhibited an impairment of antigen-induced receptor editing among both the endogenous lambda and kappa loci in vitro and in vivo. Importantly, PLCgamma2 deficiency impaired BCR-induced expression of IRF-4 and IRF-8, the two transcription factors critical for lambda and kappa light-chain rearrangements. Taken together, these data demonstrate that the PLCgamma2 signaling pathway plays a role in activation of light-chain loci and contributes to receptor editing.

Author List

Bai L, Chen Y, He Y, Dai X, Lin X, Wen R, Wang D

Authors

Demin Wang PhD Assistant Professor in the Microbiology and Immunology department at Medical College of Wisconsin
Renren Wen PhD Associate Investigator in the Blood Research Institute department at BloodCenter of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Apoptosis
B-Lymphocytes
Cell Proliferation
Gene Expression Regulation
Gene Rearrangement, B-Lymphocyte
Immunoglobulin Light Chains
Interferon Regulatory Factors
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Phospholipase C gamma
Pre-B Cell Receptors
Signal Transduction
Transcriptional Activation
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