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Activation of the heat shock transcription factor by hypoxia in mammalian cells. Proc Natl Acad Sci U S A 1990 Aug;87(16):6263-7

Date

08/01/1990

Pubmed ID

1696723

Pubmed Central ID

PMC54513

DOI

10.1073/pnas.87.16.6263

Scopus ID

2-s2.0-0024999067   153 Citations

Abstract

Members of the stress protein family such as HSP70 are induced in ischemic tissues and may contribute to the ability of cells to survive episodes of transient circulatory insufficiency. However, the biochemical events that lead to this induction, and their degree of similarity with pathways triggered by heat stress, have not been defined. In this study, we demonstrate that transient exposure of cultured C2C12 mouse myogenic cells to a hypoxic atmosphere stimulates DNA binding activity of the heat shock transcription factor through mechanisms that are independent of new protein synthesis. Activation of heat shock transcription factor in hypoxic cells is temporally associated with induction of endogenous HSP70 gene transcription and with induction of a heterologous reporter gene controlled by the human HSP70 promoter. Furthermore, induction of the human HSP70 promoter by hypoxia requires an intact heat shock element, indicating that other cis-acting transcriptional control elements contained within this complex promoter are not sufficient to transduce signals generated within hypoxic cells. These findings provide strong evidence that hypoxia and heat shock induce expression of the HSP70 gene by similar, if not identical, mechanisms.

Author List

Benjamin IJ, Kröger B, Williams RS

Author

Ivor J. Benjamin MD Center Director, Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Anaerobiosis
Animals
Base Sequence
Cell Line
Cycloheximide
Gene Expression
Heat-Shock Proteins
Hot Temperature
Hypoxia
Kinetics
Molecular Sequence Data
Mutation
Oligodeoxyribonucleotides
Promoter Regions, Genetic
RNA
Transcription Factors
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a