Faculty Collaboration Database

Far red/near infrared light treatment promotes femoral artery collateralization in the ischemic hindlimb. J Mol Cell Cardiol 2013 Sep;62:36-42

Date

05/25/2013

Pubmed ID

23702287

Pubmed Central ID

PMC3747970

DOI

10.1016/j.yjmcc.2013.05.007

Scopus ID

2-s2.0-84878680394 (requires institutional sign-in at Scopus site)   19 Citations

Abstract

Nitric oxide (NO) is a crucial mediator of hindlimb collateralization and angiogenesis. Within tissues there are nitrosyl-heme proteins which have the potential to generate NO under conditions of hypoxia or low pH. Low level irradiation of blood and muscle with light in the far red/near infrared spectrum (670 nm, R/NIR) facilitates NO release. Therefore, we assessed the impact of red light exposure on the stimulation of femoral artery collateralization. Rabbits and mice underwent unilateral resection of the femoral artery and chronic R/NIR treatment. The direct NO scavenger carboxy-PTIO and the nitric oxide synthase (NOS) inhibitor L-NAME were also administered in the presence of R/NIR. DAF fluorescence assessed R/NIR changes in NO levels within endothelial cells. In vitro measures of R/NIR induced angiogenesis were assessed by endothelial cell proliferation and migration. R/NIR significantly increased collateral vessel number which could not be attenuated with L-NAME. R/NIR induced collateralization was abolished with c-PTIO. In vitro, NO production increased in endothelial cells with R/NIR exposure, and this finding was independent of NOS inhibition. Similarly R/NIR induced proliferation and tube formation in a NO dependent manner. Finally, nitrite supplementation accelerated R/NIR collateralization in wild type C57Bl/6 mice. In an eNOS deficient transgenic mouse model, R/NIR restores collateral development. In conclusion, R/NIR increases NO levels independent of NOS activity, and leads to the observed enhancement of hindlimb collateralization.

Author List

Lohr NL, Ninomiya JT, Warltier DC, Weihrauch D

Author

Dorothee Weihrauch DVM, PhD Research Scientist II in the Anesthesiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Cell Proliferation
Femoral Artery
Hindlimb
Human Umbilical Vein Endothelial Cells
Humans
Ischemia
Light
Mice
Mice, Inbred C57BL
Mice, Knockout
Neovascularization, Physiologic
Nitric Oxide
Rabbits