Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

The T cell activation marker CD150 can be used to identify alloantigen-activated CD4(+)25+ regulatory T cells. Cell Immunol 2004 Feb;227(2):129-39

Date

05/12/2004

Pubmed ID

15135295

DOI

10.1016/j.cellimm.2004.03.003

Scopus ID

2-s2.0-2342634326   16 Citations

Abstract

We have been investigating whether alloantigen-specific CD4(+)25+ regulatory T cells can be identified for use in treating graft-versus-host disease. CD150, which is upregulated on the surface of all activated T lymphocytes, was identified as a candidate marker for alloantigen-activated CD4(+)25+ regulatory T cells by gene chip analysis. Freshly isolated CD4(+)25+ cells had only low cell-surface expression of CD150, comparable to that of CD4(+)25- T cells. Increased CD150 expression was observed on all T cells after coculture with allogeneic stimulator cells. When purified CD4(+)25+ cells were precultured with allogeneic stimulator cells, then sorted into CD150+ and CD150- subsets, allosuppressive activity was contained primarily in the CD150+ fraction. These cells also suppressed the proliferation of alloantigen-activated autologous T cells, and they could be expanded in vitro without loss of their suppressive capacity. These results suggest that CD150 can be used as a marker for the identification of purified alloantigen-activated CD4(+)25+ regulatory T cells.

Author List

Browning MB, Woodliff JE, Konkol MC, Pati NT, Ghosh S, Truitt RL, Johnson BD

Authors

Meghen B. Browning MD Associate Professor in the Pediatrics department at Medical College of Wisconsin
Bryon D. Johnson PhD Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Antigens, CD
CD4 Antigens
Glycoproteins
Humans
Immunoglobulins
Isoantigens
Lymphocyte Activation
Lymphocyte Culture Test, Mixed
Receptors, Antigen, T-Cell
Receptors, Cell Surface
Receptors, Interleukin-2
Signaling Lymphocytic Activation Molecule Family Member 1
T-Lymphocytes