Expression of cytochrome P450-4A isoforms in the rat cremaster muscle microcirculation. Microcirculation 2004;11(1):89-96
Date
07/29/2004Pubmed ID
15280100DOI
10.1080/10739680490266225Scopus ID
2-s2.0-10244238855 (requires institutional sign-in at Scopus site) 10 CitationsAbstract
OBJECTIVE: This study sought to identify any specific cytochrome P450 (CYP450) -4A enzyme isoforms expressed in arterioles and/or the surrounding parenchymal tissue of the rat cremaster muscle.
METHODS: RT-PCR was used to detect the presence of specific CYP450-4A isoforms in isolated muscle fibers and arterioles from the cremaster muscle of Sprague-Dawley rats; CYP450-4A protein expression was determined by Western blotting.
RESULTS: CYP450-4A3 mRNA was expressed in isolated muscle fibers and in cremasteric arterioles, while CYP450-4A8 mRNA was expressed only in cremasteric arterioles. CYP450-4A1 and CYP450-4A2 mRNA were not expressed in arterioles and skeletal muscle cells, although all four isoforms were strongly expressed in the liver. CYP450-4A protein was detected in both the isolated muscle fibers and in the isolated arterioles.
CONCLUSIONS: The present study identifies the specific pattern of cytochrome P450-4A isoform expression in arterioles and parenchymal cells of the skeletal muscle microcirculation, and supports the hypothesis that the cytochrome P-450 enzymes may play a role in the regulation of microvascular function in the skeletal muscle microcirculation.
Author List
Wang J, Maier KG, Roman RJ, De La Cruz L, Zhu J, Henderson L, Lombard JHMESH terms used to index this publication - Major topics in bold
AnimalsArterioles
Cytochrome P-450 CYP4A
Cytochrome P-450 Enzyme System
Cytochrome P450 Family 4
Isoenzymes
Male
Microcirculation
Muscle Fibers, Skeletal
Muscle, Skeletal
RNA, Messenger
Rats
Rats, Sprague-Dawley
Tissue Distribution