Faculty Collaboration Database

Facile synthesis, characterization, and antimicrobial activity of cellulose-chitosan-hydroxyapatite composite material: a potential material for bone tissue engineering. J Biomed Mater Res A 2013 Nov;101(11):3266-77

Date

04/19/2013

Pubmed ID

23595871

Pubmed Central ID

PMC3788024

DOI

10.1002/jbm.a.34636

Scopus ID

2-s2.0-84884904754 (requires institutional sign-in at Scopus site)   53 Citations

Abstract

Hydroxyapatite (HAp) is often used as a bone-implant material because it is biocompatible and osteoconductive. However, HAp possesses poor rheological properties and it is inactive against disease-causing microbes. To improve these properties, we developed a green method to synthesize multifunctional composites containing: (1) cellulose (CEL) to impart mechanical strength; (2) chitosan (CS) to induce antibacterial activity thereby maintaining a microbe-free wound site; and (3) HAp. In this method, CS and CEL were co-dissolved in an ionic liquid (IL) and then regenerated from water. HAp was subsequently formed in situ by alternately soaking [CEL+CS] composites in aqueous solutions of CaCl2 and Na2 HPO4 . At least 88% of IL used was recovered for reuse by distilling the aqueous washings of [CEL+CS]. The composites were characterized using FTIR, XRD, and SEM. These composites retained the desirable properties of their constituents. For example, the tensile strength of the composites was enhanced 1.9 times by increasing CEL loading from 20% to 80%. Incorporating CS in the composites resulted in composites which inhibited the growth of both Gram positive (MRSA, S. aureus and VRE) and Gram negative (E. coli and P. aeruginosa) bacteria. These findings highlight the potential use of [CEL+CS+HAp] composites as scaffolds in bone tissue engineering.

Author List

Mututuvari TM, Harkins AL, Tran CD

Authors

April Harkins PhD Assistant Professor in the Clinical Laboratory Science department at Marquette University
Chieu Tran PhD Plfetschinger-Habermann Professor in the Chemistry department at Marquette University

MESH terms used to index this publication - Major topics in bold

Anti-Bacterial Agents
Biocompatible Materials
Bone and Bones
Cellulose
Chitosan
Durapatite
Escherichia coli
Microbial Sensitivity Tests
Microscopy, Electron, Scanning
Photoelectron Spectroscopy
Pseudomonas aeruginosa
Spectrophotometry, Atomic
Spectroscopy, Fourier Transform Infrared
Spectroscopy, Near-Infrared
Tensile Strength
Tissue Engineering
X-Ray Diffraction