Faculty Collaboration Database

Cytotoxicity of endodontic materials. J Endod 1998 Feb;24(2):91-6

Date

06/26/1998

Pubmed ID

9641138

DOI

10.1016/S0099-2399(98)80084-8

Scopus ID

2-s2.0-0031990071 (requires institutional sign-in at Scopus site)   181 Citations

Abstract

An in vitro cell culture model of human gingival fibroblasts and L-929 cells was used to measure the cytotoxicity of currently used root canal sealers Endomet, CRCS, and AH26 and root-end filling materials Amalgam, Gallium GF2, Ketac Silver, mineral trioxide aggregate (MTA), and Super-EBA. Cytotoxic effects were assessed using the MTT assay for mitochondrial enzyme activity and the CV assay for cell numbers. Using inserts culture and L-929 fibroblasts. All-Bond-2 was also evaluated. The statistical analysis of results showed that CRCS was the least cytotoxic sealer followed by Endomet and AH26. Among root-end filling materials, MTA was not cytotoxic; Gallium GF2 displayed little cytotoxicity; and Ketac Silver, Super-EBA, and Amalgam showed higher levels of cytotoxicity. All Bond-2 also displayed a high degree of cytotoxicity. CRCS was the best root canal sealer and MTA the best root-end filling material. The outcome was favorable also for Gallium GF2 as a retrofilling material.

Author List

Osorio RM, Hefti A, Vertucci FJ, Shawley AL

Author

Arthur Hefti DDS,PhD Associate Dean - Research & Graduate Studies in the Dentistry department at Marquette University

MESH terms used to index this publication - Major topics in bold

Alloys
Aluminum Compounds
Analysis of Variance
Animals
Bismuth
Calcium Compounds
Calcium Hydroxide
Cells, Cultured
Cermet Cements
Dental Alloys
Dentin-Bonding Agents
Drug Combinations
Epoxy Resins
Evaluation Studies as Topic
Fibroblasts
Gallium
Gingiva
Humans
Methacrylates
Methenamine
Mice
Oxides
Retrograde Obturation
Root Canal Filling Materials
Silicates
Silver
Titanium
Zinc Oxide
Zinc Oxide-Eugenol Cement