Optical imaging of mitochondrial redox state in rodent model of retinitis pigmentosa. J Biomed Opt 2013 Jan;18(1):16004
Date
01/08/2013Pubmed ID
23291617Pubmed Central ID
PMC3537487DOI
10.1117/1.JBO.18.1.016004Scopus ID
2-s2.0-84878225544 (requires institutional sign-in at Scopus site) 23 CitationsAbstract
Oxidative stress (OS) and mitochondrial dysfunction contribute to photoreceptor cell loss in retinal degenerative disorders. The metabolic state of the retina in a rodent model of retinitis pigmentosa (RP) was investigated using a cryo-fluorescence imaging technique. The mitochondrial metabolic coenzymes nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) are autofluorescent and can be monitored without exogenous labels using optical techniques. The cryo-fluorescence redox imaging technique provides a quantitative assessment of the metabolism. More specifically, the ratio of the fluorescence intensity of these fluorophores (NADH/FAD), the NADH redox ratio (RR), is a marker of the metabolic state of the tissue. The NADH RR and retinal function were examined in an established rodent model of RP, the P23H rat compared to that of nondystrophic Sprague-Dawley (SD) rats. The NADH RR mean values were 1.11 ± 0.03 in the SD normal and 0.841 ± 0.01 in the P23H retina, indicating increased OS in the P23H retina. Electroretinographic data revealed a significant reduction in photoreceptor function in P23H animals compared to SD nozrmal rats. Thus, cryo-fluorescence redox imaging was used as a quantitative marker of OS in eyes from transgenic rats and demonstrated that alterations in the oxidative state of eyes occur during the early stages of RP.
Author List
Maleki S, Gopalakrishnan S, Ghanian Z, Sepehr R, Schmitt H, Eells J, Ranji MAuthors
Janis Eells PhD Professor in the Biomedical Sciences department at University of Wisconsin - MilwaukeeSandeep Gopalakrishnan MS, PhD Assistant Professor and Director, Biobehavioral Research Laboratory in the College of Nursing department at University of Wisconsin - Milwaukee
Mahsa Ranji PhD Assistant Professor in the Electrical Engineering department at University of Wisconsin - Milwaukee
MESH terms used to index this publication - Major topics in bold
Analysis of VarianceAnimals
Disease Models, Animal
Image Processing, Computer-Assisted
Mitochondria
NAD
Optical Imaging
Oxidation-Reduction
Oxidative Stress
Rats
Rats, Sprague-Dawley
Rats, Transgenic
Retinitis Pigmentosa