Faculty Collaboration Database

Effects of low-level light therapy on hepatic antioxidant defense in acute and chronic diabetic rats. J Biochem Mol Toxicol 2009;23(1):1-8

Date

02/10/2009

Pubmed ID

19202557

DOI

10.1002/jbt.20257

Scopus ID

2-s2.0-59949099120 (requires institutional sign-in at Scopus site)   30 Citations

Abstract

Diabetes causes oxidative stress in the liver and other tissues prone to complications. Photobiomodulation by near infrared light (670 nm) has been shown to accelerate diabetic wound healing, improve recovery from oxidative injury in the kidney, and attenuate degeneration in retina and optic nerve. The present study tested the hypothesis that 670 nm photobiomodulation, a low-level light therapy, would attenuate oxidative stress and enhance the antioxidant protection system in the liver of a model of type I diabetes. Male Wistar rats were made diabetic with streptozotocin (50 mg/kg, ip) then exposed to 670 nm light (9 J/cm(2)) once per day for 18 days (acute) or 14 weeks (chronic). Livers were harvested, flash frozen, and then assayed for markers of oxidative stress. Light treatment was ineffective as an antioxidant therapy in chronic diabetes, but light treatment for 18 days in acutely diabetic rats resulted in the normalization of hepatic glutathione reductase and superoxide dismutase activities and a significant increase in glutathione peroxidase and glutathione-S transferase activities. The results of this study suggest that 670 nm photobiomodulation may reduce, at least in part, acute hepatic oxidative stress by enhancing the antioxidant defense system in the diabetic rat model.

Author List

Lim J, Ali ZM, Sanders RA, Snyder AC, Eells JT, Henshel DS, Watkins JB 3rd

Author

Janis Eells PhD Professor in the Biomedical Sciences department at University of Wisconsin - Milwaukee

MESH terms used to index this publication - Major topics in bold

Acute Disease
Animals
Antioxidants
Blood Glucose
Body Weight
Chronic Disease
Diabetes Mellitus, Experimental
Glutathione
Glutathione Peroxidase
Glutathione Reductase
Glutathione Transferase
Lipid Peroxidation
Liver
Male
Malondialdehyde
Phototherapy
Rats
Rats, Wistar
Superoxide Dismutase