3.6 kb genomic sequence from Takifugu capable of promoting axon growth-associated gene expression in developing and regenerating zebrafish neurons. Gene Expr Patterns 2008 Jul;8(6):382-388
Date
07/05/2008Pubmed ID
18599366Pubmed Central ID
PMC2603057DOI
10.1016/j.gep.2008.05.002Scopus ID
2-s2.0-46749132631 (requires institutional sign-in at Scopus site) 36 CitationsAbstract
Unlike mammals, fish have the capacity for functional adult CNS regeneration, which is due, in part, to their ability to express axon growth-related genes in response to nerve injury. One such axon growth-associated gene is gap43, which is expressed during periods of developmental and regenerative axon growth, but is not expressed in CNS neurons that do not regenerate in adult mammals. We previously demonstrated that cis-regulatory elements of gap43 that are sufficient for developmental expression are not sufficient for regenerative expression in the zebrafish. Here we have identified a 3.6kb genomic sequence from Fugu rubripes that can promote reporter gene expression in the nervous system during both development and regeneration in zebrafish. This compact sequence is advantageous for functional dissection of regions important for axon growth-associated gene expression during development and/or regeneration. In addition, this sequence will also be useful for targeting gene expression to neurons during periods of growth and plasticity.
Author List
Udvadia AJAuthor
Ava Udvadia BS,PhD Associate Professor in the Biological Sciences department at University of Wisconsin - MilwaukeeMESH terms used to index this publication - Major topics in bold
Amino Acid SequenceAnimals
Animals, Genetically Modified
Axons
Enhancer Elements, Genetic
Evolution, Molecular
Fish Proteins
GAP-43 Protein
Gene Expression
Genome
Molecular Sequence Data
Nerve Regeneration
Nervous System
Neurons
Promoter Regions, Genetic
Retina
Sequence Homology, Amino Acid
Takifugu
Zebrafish
