Faculty Collaboration Database

Sp-1 binds promoter elements regulated by the RB protein and Sp-1-mediated transcription is stimulated by RB coexpression. Proc Natl Acad Sci U S A 1993 Apr 15;90(8):3265-9

Date

04/15/1993

Pubmed ID

8475068

Pubmed Central ID

PMC46280

DOI

10.1073/pnas.90.8.3265

Scopus ID

2-s2.0-0027480441 (requires institutional sign-in at Scopus site)   198 Citations

Abstract

The retinoblastoma (RB) protein is implicated in transcriptional regulation of at least five cellular genes, including c-fos, c-myc, and transforming growth factor beta 1. Cotransfection of RB and truncated promoter constructs has defined a discrete element (retinoblastoma control element; RCE) within the promoters of each of these genes as being necessary for RB-mediated transcription control. Previously, we have shown that RCEs form protein-DNA complexes in vitro with three heretofore unidentified nuclear proteins and mutation of their DNA-binding site within the c-fos RCE results in an abrogation of RCE-dependent transcription in vivo. Here, we demonstrate that one of the nuclear proteins that binds the c-fos, c-myc, and transforming growth factor beta 1 RCEs in vitro is Sp-1 and that Sp-1 stimulates RCE-dependent transcription in vivo. Moreover, we show that Sp-1-mediated transcription is stimulated by the transient coexpression of RB protein. We conclude from these observations that RB may regulate transcription in part by virtue of its ability to functionally interact with Sp-1.

Author List

Udvadia AJ, Rogers KT, Higgins PD, Murata Y, Martin KH, Humphrey PA, Horowitz JM

Author

Ava Udvadia BS,PhD Associate Professor in the Biological Sciences department at University of Wisconsin - Milwaukee

MESH terms used to index this publication - Major topics in bold

3T3 Cells
Animals
Base Sequence
Binding Sites
Blotting, Western
Cell Line
Drosophila
Gene Expression Regulation
Genes, Retinoblastoma
Genes, fos
Genes, myc
Humans
Mice
Molecular Sequence Data
Nuclear Proteins
Oligodeoxyribonucleotides
Promoter Regions, Genetic
Retinoblastoma Protein
Sp1 Transcription Factor
Substrate Specificity
Transcription, Genetic
Transfection
Transforming Growth Factor beta
Tumor Cells, Cultured