Faculty Collaboration Database

Role of sustained antigen release from nanoparticle vaccines in shaping the T cell memory phenotype. Biomaterials 2012 Jun;33(19):4957-64

Date

04/10/2012

Pubmed ID

22484047

Pubmed Central ID

PMC5724530

DOI

10.1016/j.biomaterials.2012.03.041

Scopus ID

2-s2.0-84859838781 (requires institutional sign-in at Scopus site)   243 Citations

Abstract

Particulate vaccines are emerging promising technologies for the creation of tunable prophylactics against a wide variety of conditions. Vesicular and solid biodegradable polymer platforms, exemplified by liposomes and polyesters, respectively, are two of the most ubiquitous platforms in vaccine delivery studies. Here we directly compared the efficacy of each in a long-term immunization study and in protection against a model bacterial antigen. Immunization with poly(lactide-co-glycolide) (PLGA) nanoparticles elicited prolonged antibody titers compared to liposomes and alum. The magnitude of the cellular immune response was also highest in mice vaccinated with PLGA, which also showed a higher frequency of effector-like memory T cell phenotype, leading to an effective clearance of intracellular bacteria. The difference in performance of these two common particulate platforms is shown not to be due to material differences but appears to be connected to the kinetics of antigen delivery. Thus, this study highlights the importance of sustained antigen release mediated by particulate platforms and its role in the long-term appearance of effector memory cellular response.

Author List

Demento SL, Cui W, Criscione JM, Stern E, Tulipan J, Kaech SM, Fahmy TM

MESH terms used to index this publication - Major topics in bold

Animals
Antigens
Female
Lactic Acid
Liposomes
Mice
Mice, Inbred BALB C
Nanoparticles
Polyglycolic Acid
T-Lymphocytes
Vaccines