Faculty Collaboration Database

Lesion-induced increase in survival and migration of human neural progenitor cells releasing GDNF. Cell Transplant 2008;17(7):753-62

Date

12/03/2008

Pubmed ID

19044202

Pubmed Central ID

PMC2650839

DOI

10.3727/096368908786516819

Scopus ID

2-s2.0-58149198246 (requires institutional sign-in at Scopus site)   44 Citations

Abstract

The use of human neural progenitor cells (hNPC) has been proposed to provide neuronal replacement or astrocytes delivering growth factors for brain disorders such as Parkinson's and Huntington's disease. Success in such studies likely requires migration from the site of transplantation and integration into host tissue in the face of ongoing damage. In the current study, hNPC modified to release glial cell line-derived neurotrophic factor (hNPCGDNF) were transplanted into either intact or lesioned animals. GDNF release itself had no effect on the survival, migration, or differentiation of the cells. The most robust migration and survival was found using a direct lesion of striatum (Huntington's model) with indirect lesions of the dopamine system (Parkinson's model) or intact animals showing successively less migration and survival. No lesion affected differentiation patterns. We conclude that the type of brain injury dictates migration and integration of hNPC, which has important consequences when considering transplantation of these cells as a therapy for neurodegenerative diseases.

Author List

Behrstock S, Ebert AD, Klein S, Schmitt M, Moore JM, Svendsen CN

Author

Allison D. Ebert PhD Associate Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Cell Differentiation
Cell Movement
Cell Survival
Cells, Cultured
Corpus Striatum
Glial Cell Line-Derived Neurotrophic Factor
Humans
Neurodegenerative Diseases
Neurons
Stem Cell Transplantation
Stem Cells